Media Selction guide


DMEM

Cat
D6429, D5796, D5671, D6171, D6046, D5546
Media
DMEM High glucose / DMEM Low glucose
glucose
4500 mg/L or 1000 mg/L
L-glutamine
Yes
Phenol red
Yes
NaHCO₃
Yes
Hepes
Yes (only D6171)
Size
500ml, 1L, 24×500ml (varies by item)

RPMI 1640

CatR8758, R0883, R7388
MediaRPMI1640
glucose2000 mg/L
L-glutamineYes
Phenol redYes
NaHCO₃Yes
HepesYes (R7388 only)
Size500ml, 1L, 6×500ml

MEM, MEMα

CatM4655, M2279
MediaMEM/EBSS
glucose1000 mg/L
L-glutamineYes
Phenol redYes
NaHCO₃Yes
Hepes
Earle’s saltsYes
Size500ml

IMDM

CatI6529
MediaIMDM
glucose4500 mg/L
L-glutamineYes
Phenol redYes
NaHCO₃Yes
HepesYes
Size500ml

M199

<M199>
CatM4530, M2154
MediaMedium 199
glucose1000 mg/L
L-glutamineYes
Phenol redYes
NaHCO₃Yes
HepesYes
Earle’s saltsYes
Size500ml

Supplements


Antibiotics

Cat품명속성size
P0781Penicillin-Streptomycin, 100x10,000 units penicillin, 10 mg streptomycin per mL in 0.9% NaCl, 0.1 μm filtered100ml
P4333Penicillin-Streptomycin, 100x10,000 units penicillin and 10 mg streptomycin/mL 0.1 μm filtered100ml
A5955Antibiotic Antimycotic Solution (100×), Stabilized10,000 units penicillin, 10 mg streptomycin and 25 μg amphotericin B per mL, 0.1 μm filtered100ml
G1272Gentamicin solution10 mg/mL in deionized water50ml
P9620Puromycin dihydrochloride10 mg/mL in H₂O10ml

Cell Dissociation

<Cell Dissociation>
Cat품명속성size
T4049Trypsin-EDTA solution, 0.25%porcine trypsin, EDTA, HBSS, Phenol red100ml, 500ml
T3924Trypsin-EDTA solution, 1Xporcine trypsin, EDTA, HBSS, Phenol red100ml, 500ml

Freezing Cell

<Freezing Cell>
Cat품명속성size
D2650Dimethyl sulfoxidesterile-filtered, suitable for hybridoma, ≥99.7%5×5ml, 100ml